ABSTRACT
Objective To explore the effect of OASL expression on the proliferation and migration of pancreatic cancer cells. Methods The GEPIA database was used to analyze the differences in OASL expression in pancreatic cancer tissues and normal pancreatic tissues. The TIMER database was used to analyze the relationship between OASL expression and patient survival. The TCGA database was used to analyze the correlation of OASL expression with the clinicopathological parameters of pancreatic cancer. shRNA was used to knock down the expression of OASL gene in pancreatic cancer panc-1 cells. Lentiviruses were used to overexpress the OASL gene in pancreatic cancer cells. MTT assay was used to evaluate their proliferation ability, and scratch and Transwell experiments were used to evaluate their migration ability. Western blot experiments were used to detect changes in proteins related to tumor proliferation, migration, and invasion. Results OASL expression in the pancreatic cancer group was significantly higher than that in normal pancreatic tissue (P < 0.05), and patients with high OASL expression in pancreatic cancer patients had worse OS than patients with low expression (P < 0.05). After OASL gene knockdown, the proliferation and migration abilities of panc-1 cells were inhibited, whereas the overexpression of OASL gene promoted the proliferation and migration ability of panc-1 cells. Western blot experiments showed that after OASL knockdown, p-STAT3 protein expression increased, whereas STAT3 and BAK protein expressions decreased. After OASL overexpression, p-STAT3 protein expression decreased, and STAT3 and BAK protein expression increased. Conclusion OASL may affect the proliferation and migration of pancreatic cancer cells through the STAT3 signaling pathway while affecting BAK expression to induce cell death.
ABSTRACT
Objective To investigate the impact of mind wandering in sustained attention response task, and offer interventions to cope with mind wandering. Methods In December, 2016, a total of 37 athletes were tested with sustained attention response task. Their technical index, reaction time variation and accurate rate were calculated. Results The statistics of 25 athletes showed that, the technical index was lower in complex task than in simple task (t(35)=-8.019, P<0.001). No significant difference was found in the reaction time variation between two tasks (t(23)=-1.575, P=0.129), the main effect of mind wandering was not significant (F(1,23)=0.488, P=0.492), as well as the interaction of mind wandering and task difficulty (F(1,23)=0.054, P=0.262). No significant difference was found in the accurate rate between two tasks (t(23)=-1.046, P=0.307), the main effect of mind wandering was not significant (F(1,23)=0.119, P=0.733), and the interaction of mind wander-ing and task difficulty was marginal significance (F(1,23)=3.608, P=0.070). Conclusion Athlete's mind wandering with awareness would influ-ence the performance in complex sustained attention response task. Mindfulness training and tasks engagement could effectively cope with mind wandering.